SARS Coronavirus Detection Methods
Identifieur interne : 004669 ( Main/Exploration ); précédent : 004668; suivant : 004670SARS Coronavirus Detection Methods
Auteurs : Susanna K. P. Lau [République populaire de Chine] ; Xiao-Yan Che [République populaire de Chine] ; Patrick C. Y. Woo [République populaire de Chine] ; Beatrice H. L. Wong [République populaire de Chine] ; Vincent C. C. Cheng [République populaire de Chine] ; Gibson K. S. Woo [République populaire de Chine] ; Ivan F. N. Hung [République populaire de Chine] ; Rosana W. S. Poon [République populaire de Chine] ; Kwok-Hung Chan [République populaire de Chine] ; J. S. Malik Peiris [République populaire de Chine] ; Kwok-Yung Yuen [République populaire de Chine]Source :
- Emerging Infectious Diseases [ 1080-6040 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- isolement et purification : Protéines nucléocapside, Virus du SRAS.
- virologie : Fèces.
- Humains, RT-PCR, Sensibilité et spécificité, Syndrome respiratoire aigu sévère, Test ELISA.
English descriptors
- KwdEn :
- Enzyme-Linked Immunosorbent Assay (methods), Feces (virology), Humans, Nucleocapsid Proteins (isolation & purification), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (urine).
- MESH :
- chemical , isolation & purification : Nucleocapsid Proteins.
- diagnosis : Severe Acute Respiratory Syndrome.
- isolation & purification : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay, Reverse Transcriptase Polymerase Chain Reaction.
- urine : Severe Acute Respiratory Syndrome.
- virology : Feces.
- Humans, Sensitivity and Specificity.
Abstract
Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.
Url:
DOI: 10.3201/eid1107.041045
PubMed: 16022791
PubMed Central: 3371792
Affiliations:
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Le document en format XML
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Lau</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="3"><nlm:aff id="aff2">First Military Medical University, Guangzhou, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>First Military Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. 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Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">16022791</idno><idno type="pmc">3371792</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3371792</idno><idno type="RBID">PMC:3371792</idno><idno type="doi">10.3201/eid1107.041045</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000866</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000866</idno><idno type="wicri:Area/Pmc/Curation">000866</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000866</idno><idno type="wicri:Area/Pmc/Checkpoint">001252</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001252</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:16022791</idno><idno type="wicri:Area/PubMed/Corpus">002640</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002640</idno><idno type="wicri:Area/PubMed/Curation">002640</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002640</idno><idno type="wicri:Area/PubMed/Checkpoint">002525</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002525</idno><idno type="wicri:Area/Ncbi/Merge">001061</idno><idno type="wicri:Area/Ncbi/Curation">001061</idno><idno type="wicri:Area/Ncbi/Checkpoint">001061</idno><idno type="wicri:doubleKey">1080-6040:2005:Lau S:sars:coronavirus:detection</idno><idno type="wicri:Area/Main/Merge">004935</idno><idno type="wicri:Area/Main/Curation">004669</idno><idno type="wicri:Area/Main/Exploration">004669</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">SARS Coronavirus Detection Methods</title><author><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. 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Lau</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation wicri:level="3"><nlm:aff id="aff2">First Military Medical University, Guangzhou, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>First Military Medical University, Guangzhou</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Woo, Gibson K S" sort="Woo, Gibson K S" uniqKey="Woo G" first="Gibson K. S." last="Woo">Gibson K. S. Woo</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name><affiliation wicri:level="1"><nlm:aff id="aff3">Queen Mary Hospital, Hong Kong Special Administrative Region, People's Republic of China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Queen Mary Hospital, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W. S." last="Poon">Rosana W. S. 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Malik Peiris</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author><author><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name><affiliation wicri:level="1"><nlm:aff id="aff1">University of Hong Kong, Hong Kong Special Administrative Region, People's Republic of China;</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>University of Hong Kong, Hong Kong Special Administrative Region</wicri:regionArea><wicri:noRegion>Hong Kong Special Administrative Region</wicri:noRegion></affiliation></author></analytic><series><title level="j">Emerging Infectious Diseases</title><idno type="ISSN">1080-6040</idno><idno type="eISSN">1080-6059</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Feces (virology)</term><term>Humans</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (urine)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Fèces (virologie)</term><term>Humains</term><term>Protéines nucléocapside (isolement et purification)</term><term>RT-PCR ()</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (urine)</term><term>Test ELISA ()</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="urine" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Fèces</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Feces</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Humans</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Syndrome respiratoire aigu sévère</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Using clinical samples from patients with severe acute respiratory syndrome, we showed that the sensitivities of a quantitative reverse transcription–polymerase chain reaction (80% for fecal samples and 25% for urine samples) were higher than those of the polyclonal (50% and 5%) and monoclonal (35% and 8%) antibody-based nucleocapsid antigen capture enzyme-linked immunosorbent assays.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><noRegion><name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K. P." last="Lau">Susanna K. P. Lau</name></noRegion><name sortKey="Chan, Kwok Hung" sort="Chan, Kwok Hung" uniqKey="Chan K" first="Kwok-Hung" last="Chan">Kwok-Hung Chan</name><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><name sortKey="Cheng, Vincent C C" sort="Cheng, Vincent C C" uniqKey="Cheng V" first="Vincent C. C." last="Cheng">Vincent C. C. Cheng</name><name sortKey="Hung, Ivan F N" sort="Hung, Ivan F N" uniqKey="Hung I" first="Ivan F. N." last="Hung">Ivan F. N. Hung</name><name sortKey="Peiris, J S Malik" sort="Peiris, J S Malik" uniqKey="Peiris J" first="J. S. Malik" last="Peiris">J. S. Malik Peiris</name><name sortKey="Poon, Rosana W S" sort="Poon, Rosana W S" uniqKey="Poon R" first="Rosana W. S." last="Poon">Rosana W. S. Poon</name><name sortKey="Wong, Beatrice H L" sort="Wong, Beatrice H L" uniqKey="Wong B" first="Beatrice H. L." last="Wong">Beatrice H. L. Wong</name><name sortKey="Woo, Gibson K S" sort="Woo, Gibson K S" uniqKey="Woo G" first="Gibson K. S." last="Woo">Gibson K. S. Woo</name><name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C. Y." last="Woo">Patrick C. Y. Woo</name><name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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